Officials from Afghanistan Chamber of Commerce and Industries (ACCI) on Monday said Islamabad again closed its doors to Afghan apple traders.
The officials have expressed concern that if this continues, thousands of tons of apples will go spoilt.
Pakistan claims that the reason for this ban on apply import from Afghanistan is because they believe they are Iranian apples.
The decision by Pakistan comes just as Afghanistan reaches its apple harvesting season.
The Fresh Fruits Exporters Union officials said if Pakistan routes are not re-opened for Afghan apple trade, farmers in Maidan Wardak, Ghazni and Baghlan provinces will suffer heavy losses.
So far there has not been any comment by any government official from Ministry of Industries and Commerce, or anyone else.